NucPE1 (Nuclear Peroxy Emerald 1) 是核定位荧光过氧化氢探针, 其特异性定位于细胞核，没有附加的靶向部分。

NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

Solid

NucPE1 features two major visible region absorptions (λabs=468 nm, ε=27,300 Mcm; λabs=490 nm, ε=26,000 Mcm) and a weak emission (λem=530 nm, Φ=0.117). Reaction of NucPE1 with H2O2 triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 Mcm) with enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans , where it can respond to subcellular changes in H2O2 fluxes. has not independently confirmed the accuracy of these methods. They are for reference only.

NucPE1 maintains the ability to selectively target nuclei in vivo. NucPE1 imaging reveals a reduction in nuclear H 2 O 2 levels in worms overexpressing sir-2.1 compared to wildtype congeners, supporting a link between this longevity-promoting sirtuin protein and enhanced regulation of nuclear ROS pools. has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

[1]. Stanicka J, et al. NADPH oxidase-generated hydrogen peroxide induces DNA damage in mutant FLT3-expressing leukemia cells. J Biol Chem. 2015 Apr 10;290(15):9348-61.
[2]. Dickinson BC, et al. A nuclear-localized fluorescent hydrogen peroxide probe for monitoring sirtuin-mediated oxidative stress responses in vivo. Chem Biol. 2011 Aug 26;18(8):943-8.

In Vitro: DMSO : 25 mg/mL (51.72 mM; Need ultrasonic)配制储备液