HA14-1 是一种 Bcl-2/Bcl-x_L 拮抗剂。HA14-1 与 Bcl-2 上的特定口袋结合, IC₅₀ 约为 9 μM，并抑制 Bcl-2 功能。

HA14-1 is a Bcl-2/Bcl-X L antagonist. HA14-1 binds the designated pocket on Bcl-2 with the IC 50 of ≈9 μM in competing with the Bcl-2 binding of Flu-BakBH3, and inhibits its function.

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Bcl-2 9 μM (IC50) Bcl-xL

HA14-1 is a nonpeptidic ligand of a Bcl-2 surface pocket. HA14-1 induces the activation of Apaf-1 and caspases, possibly by binding to Bcl-2 protein and inhibiting its function. The interaction of HA14-1 with the Bcl-2 surface pocket appeared to be specific for the chemical structure of HA14-1 as a series of synthetic analogs derived from HA14-1 containing various modifications are found to have widely different Bcl-2 binding activities. To examine the effect of HA14-1 on cell viability, HL-60 cells are treated with various concentrations of HA14-1 for 4 h. HA14-1 induces the death of HL-60 cells in a dose-dependent manner. At 50 μM, HA14-1 causes the lost of viability in more than 90% of the cells. HA14-1 is a Bcl-2/Bcl-xL antagonist. has not independently confirmed the accuracy of these methods. They are for reference

Swiss nude mice are challenged with BeGBM cells (10 injected s.c.). HA14-1 (400 nmol) in 100 μL free RPMI 1640-50% DMSO, or the carrier alone, is given at the site of injection once weekly from day 2 following cell injection. HA14-1 treatment does not have any significant effect on the growth of glioblastoma tumors in immunodeficient mice as monitored twice weekly by measuring the volume of the expanding tumors. Moreover, no gross organ toxicity or weight loss can be detected in mice receiving such treatment. To analyze whether HA14-1 treatment might enhance the efficiency of another antitumoral treatment, Swiss nude mice challenged with human glioblastoma multiforme cells are also given i.p. low doses of Etoposide (2.5 mg/kg in 200 μL of 0.9% NaCl 5 days a week from day 2 following cell injection) together with HA14-1 or mock treatment. Whereas Etoposide treatment is insuffic

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[1]. Wang JL, et al. Structure-based discovery of an organic compound that binds Bcl-2 protein and induces apoptosis of tumor cells. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7124-9.
[2]. Kessel D, et al. Initiation of apoptosis and autophagy by the Bcl-2 antagonist HA14-1. Cancer Lett. 2007 May 8;249(2):294-9.

In Vitro: DMSO : ≥ 50 mg/mL (122.18 mM)配制储备液