Monochlorobimane (Chlorobimane) 是一种荧光染料 (λ_ex=380 nm，λ_em=470 nm)，用于测定细胞中谷胱甘肽 (GSH)。

Monochlorobimane (Chlorobimane) is a fluorescent dye (λ ex =380 nm, λ em =470 nm) to measure glutathione (GSH) in cellular assays.

Solid

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
    The experiment should consist of parallel negative, positive and experimental wells respectively.
    Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 μM H2O2)
2. Incubate the plate overnight at 37?°C, 5?% CO2. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of 20-100 μM from a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20°C;

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

[1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.
[2]. Manuela D. Machado, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of Applied Phycology volume 24, pages1509–1516(2012).

In Vitro: DMSO : 50 mg/mL (220.59 mM; Need ultrasonic)配制储备液