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Sulforhodamine B sodium salt

(Synonyms: 磺酰罗丹明 B; Acid Red 52; Kiton Red 620)

目录号: PL14191 纯度: ≥98%

Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。

CAS No. :3520-42-1

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PL14191-1 mL x 10 mM (in DMSO)		¥174.00	请登录

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中文名称	Sulforhodamine B sodium salt
中文别名	酸性红52;奇通红S;Sulforhodamine鍮 sodium salt;Sulforhodamine鍮;磺酰罗丹明B;丽丝胺罗丹明;食品红106;Acid Red 52 酸性红52;Food Red No. 106 食品红106;丽丝胺罗丹明B;酸性红52 标准品;酸性玫瑰红Ｂ;酸性玫瑰红Ｂ,AR;柴林红B;磺化罗丹明B;磺基罗丹明B;丽丝胺罗丹明B,Lissamine rhodamine B,BS;酸性红;酸性红52号;酸性罗丹明B,酸性红52,二甲苯红B,赤色106号,磺酰罗丹明B;酸性桃红B;磺酰罗丹明B 单钠盐;二甲苯红;酸性玫瑰红 B;柴林红 B;磺化罗丹明 B;酸性桃红 B
英文名称	Sulforhodamine B sodium salt
英文别名	Kiton Red S;sulforhodamine B monosodium salt;sulforhodamine B cell culture tested;sulforhodamine B;Acid Red 52;Food Red No. 106;ACID RED 52 SOLUTION 200ML INPLASTIC BOTTLE, LONG-TIP CAP;Lissamine rhodamine B;Sulforhodamine B Sodium Salt;SULFORHODAMINE B, WATER SOLUABLE (C.I. 45100);4'-Butoxy-3-piperidinopropiophenone hydrochloride;Acid Red 52,Sulforhodamine B monosodium salt;Dyclocaine hydrochloride;Dyclone;Dyclonine HCL;dyclonine hydrochloride;Dyclothane;dye acid red 52;Kiton Red 620;N,N,N',N'-tetraethylsulforhodamine;rhodamine 200 S sodium salt;Sulforhodamine-B;Tanaclone;Acid Red;Xylene Red;Acid rhodamine B;C.I. Acid red 52;acid leather red kb;Hydrogen 3,6-bis(diethylamino)-9-(2,4-disulphonatophenyl)xanthylium sodium salt;acid red 52;Acid Red 52;Sulforhodamine B;Sulforhodamine B sodium salt
Cas No.	3520-42-1
分子式	C₂₇H₂₉N₂O₇S₂-^.Na+
分子量	580.65
包装储存	4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
详情描述	Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。

产品详情	Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。
生物活性	Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
性状	Solid
体外研究(In Vitro)	Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive m
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
参考文献	[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

溶解度数据	In Vitro: DMSO : 50 mg/mL (86.11 mM; Need ultrasonic)H₂O : 20 mg/mL (34.44 mM; Need ultrasonic)配制储备液

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[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

Sulforhodamine B sodium salt

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