BODIPY-cholesterol 是一种带有荧光 BODIPY 基团的亲脂性和细胞通透性胆固醇类似物。BODIPY-cholesterol 可用于监测细胞内固醇摄取和细胞器间固醇通量。(激发/发射波长: 480/508 nm)。

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm).

Solid

BODIPY-cholesterol assay
To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10?μM.
Cells diluted with PBS (or other buffers) are washed (300×g, 10?min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration.
Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5?μM), mixed thoroughly and incubated for 10?-20 mins at 37?°C.
The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2?h prior to flow cytometric assessment. has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years；In solvent -80°C 6 months

[1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.
[2]. Maarit H?ltt?-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49.

In Vitro: DMSO : 1 mg/mL (1.73 mM; Need ultrasonic and warming)H₂O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)配制储备液