5(6)-Carboxyfluorescein|72088-94-9|-陌孚医药/Medlife

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5(6)-Carboxyfluorescein

目录号: PL14137 纯度: ≥99%

5(6)-Carboxyfluorescein (5(6)-FAM) 是一种胺反应性 pH 敏感的绿色荧光标记物。5(6)-Carboxyfluorescein 可用于标记蛋白质、多肽和氨基酸。5(6)-Carboxyfluorescein 可用于体内实验肿瘤位置检测。

CAS No. :72088-94-9

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中文名称	5(6)-Carboxyfluorescein
中文别名	5(6)-羧基荧光素;CF-唆基荧光素;蒎烯;5(6)-羧酸荧光素;5-(6)-羧酸荧光素;5(6)-羧基荧光素（5(6)-FAM）;5(6)-羧基荧光素);5-(6)-羧酸荧光素,异构体混合物
英文名称	5(6)-Carboxyfluorescein
英文别名	3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid;5-(AND-6)-CARBOXYFLUORESCEIN;5(6)-CARBOXYFLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;5(6)-CARBOXYFLUORESCEIN, FOR FLUORESCENC E;5(6)-FAM;3',6'-Dihydroxy-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-ar-carboxylic acid;Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo-;5(6)-Carboxyfluorescein, mixture of isomers;5(6)Carboxy fluorescein;5-(and-6)-Carboxyfluorescein mixed isomers;Fluoresceincarboxylic Acid;3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid;3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid compound with 3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid (1:1);5-(and-6)-FAM;EBD53936;5-carboxyfluorescein; carboxyfluorescein;AK685493;5(6);5(6)-Carboxyfluorescein
Cas No.	72088-94-9
分子式	C₄₂H₂₄O₁₄
分子量	752.63
包装储存	-20°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

产品详情	5(6)-Carboxyfluorescein (5(6)-FAM) 是一种胺反应性 pH 敏感的绿色荧光标记物。5(6)-Carboxyfluorescein 可用于标记蛋白质、多肽和氨基酸。5(6)-Carboxyfluorescein 可用于体内实验肿瘤位置检测。
生物活性	5(6)-Carboxyfluorescein (5(6)-FAM) is an amine-reactive pH-sensitive green fluorescent probe. 5(6)-Carboxyfluorescein (5(6)-FAM) can be used to label proteins, peptides and nucleotides. 5(6)-Carboxyfluorescein can be used for the detection of tumour areas in vivo.
性状	Solid
体外研究(In Vitro)	5(6)-Carboxyfluorescein has two main characteristics: it has two wavelengths of maximum absorbance (465 and 490 nm) and its fluorescence emission (maximum, 515 nm) increases as a function of pH in the physiological pH range of 6-7.4. has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究(In Vivo)	5(6)-Carboxyfluorescein (5 mg/kg; i.p.) can be used for in vivo pH mapping of tumor tissue. has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: CDF mice, bearing lymphoid leukemia P38
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	-20°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
参考文献	[1]. Maksim V Kvach, et al. 5(6)-carboxyfluorescein revisited: new protecting group, separation of isomers, and their spectral properties on oligonucleotides. Bioconjug Chem. Sep-Oct 2007;18(5):1691-6. [2]. Wen Ma, et al. A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback. Adv Healthc Mater. 2020 Jan;9(1):e1901100.

溶解度数据	In Vitro: DMSO : 62.5 mg/mL (83.04 mM; ultrasonic and warming and heat to 60°C)配制储备液

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1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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