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5-IAF

(Synonyms: 5-碘乙酰氨基荧光素; 5-Iodoacetamidofluorescein)

目录号: PL14152 纯度: ≥95.0%

5-IAF (5-Iodoacetamidofluorescein) 是一种吲哚乙酰胺类荧光素衍生物。5-IAF 可作为荧光探针 (fluorescent probe)，标记含有游离硫醇(半胱氨酸侧链)的蛋白质和其他分子。

CAS No. :63368-54-7

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PL14152-10mg	10mg	询价	询价
PL14152-50mg	50mg	¥2660.00	请登录

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中文名称	5-IAF
中文别名	5-碘乙酰氨基荧光素(5-IAF);4(5)-碘乙酰氨基荧光素;5-(碘乙酰胺基)荧光素;5-碘乙酰氨基荧光素
英文名称	5-IAF
英文别名	Acetamide,N-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-2-iodo-;5-(Iodoacetamido)fluorescein;5-IAF;5-IODOACETAMIDO FLUORESCEIN;5-Iodoacetamidofluorescein(5-IAF);Fluorescein-5-iodoacetamide;N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)-2-iodoacetamide;Spiro[isobenzofuran-1(3H),9'-[9H]xanthene],acetamide deriv.;5-Iodoacetamidofluorescein
Cas No.	63368-54-7
分子式	C₂₂H₁₄NO₆I
分子量	515.25
包装储存	Powder -20°C 3 years；In solvent -80°C 6 months
详情描述	5-IAF (5-Iodoacetamidofluorescein) 是一种吲哚乙酰胺类荧光素衍生物。5-IAF 可作为荧光探针 (fluorescent probe)，标记含有游离硫醇(半胱氨酸侧链)的蛋白质和其他分子。

产品详情	5-IAF (5-Iodoacetamidofluorescein) 是一种吲哚乙酰胺类荧光素衍生物。5-IAF 可作为荧光探针 (fluorescent probe)，标记含有游离硫醇(半胱氨酸侧链)的蛋白质和其他分子。
生物活性	5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).
性状	Solid
体外研究(In Vitro)	8 μM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 μM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 μM 5-IAF. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Powder -20°C 3 years；In solvent -80°C 6 months
参考文献	[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.

溶解度数据	In Vitro: DMF : 100 mg/mL (194.08 mM; ultrasonic and warming and heat to 60°C)DMSO : 50 mg/mL (97.04 mM; Need ultrasonic)配制储备液

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[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374.
[2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

5-IAF

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