Calcein Blue 是一种不渗透膜的荧光染料，是一种含有亚氨基二乙酸结构的香豆素衍生物。Calcein Blue 也是一种金属荧光变色指示剂。

Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator.

Solid

The fluorescence of Calcein Blue (CB) is quenched by the Fe ion in the Calcein Blue-Fe complex. When dopamine is added to a solution of the Calcein Blue-Fe complex, a dopamine-Fe complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe complex shows a high selectivity for dopamine among the catecholamines and related compounds examined. has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature in continental US; may vary elsewhere.

4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Em 570-590 nm Yellow

Ex 495-570 nm Green

[1]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.
[2]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.

In Vitro: DMSO : 6 mg/mL (18.68 mM; Need ultrasonic and warming)H₂O : 1 mg/mL (3.11 mM; ultrasonic and warming and heat to 80°C)配制储备液