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5-Carboxyfluorescein diacetate N-succinimidyl ester

目录号: PL12591 纯度: ≥99%

5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm，Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合，从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。

CAS No. :150206-05-6

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PL12591-10mM*1mLinDMSO	10mM*1mLinDMSO	¥1326.00	请登录

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中文名称	5-Carboxyfluorescein diacetate N-succinimidyl ester
中文别名	5-羧基二乙酰化荧光素琥珀酰亚胺酯;5-(((2,5-二氧代吡咯烷-1-基)氧基)羰基)-3-氧代-3H-螺[异苯并呋喃-1,9-氧杂蒽]-3,6-二基二乙酸酯;5-羧基荧光素二乙酸琥珀酰亚胺酯;5-羧基-荧光素二乙酸酯N-琥珀酰亚胺酯;≥90% (HPCE)
英文名称	5-Carboxyfluorescein diacetate N-succinimidyl ester
英文别名	5-(((2,5-Dioxopyrrolidin-1-yl)oxy)carbonyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate;≥90 (HPCE);5-(((2,5-Dioxopyrrolidin-1-yl)oxy)carbonyl)-3-oxo-3H-spiro-[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate;5-CARBOXYFLUORESCEIN DIACETATE N-HYDROXYSUCCINIMIDE ESTER;5-Carboxy-fluorescein diacetate N-succinimidyl ester;5-CFDA, SE [5-Carboxyfluorescein diacetate succinimidyl ester];Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-...;Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester;(2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate;5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester;CFSE;5-CFDA;5-CFDA SE;5-FAM DA SE;5-FAM DIACETATE;Diacetate-5-FAM-NHS;5-FAM-NHS DIACETATE;5-CFDA N-succinimidyl ester;CARBOXYFLUORESCEIN DIACETATE;5-CARBOXY-DI-O-ACETYLFLUORESCEIN;5-Carboxyfluorescein diacetate N-succinimidyl ester
Cas No.	150206-05-6
分子式	C₂₉H₁₉NO₁₁
分子量	557.46
包装储存	Powder -20°C 3 years；In solvent -80°C 6 months
详情描述	5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm，Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合，从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。

产品详情	5-Carboxyfluorescein diacetate N-succinimidyl ester 是一种细胞渗透性染料 (Ex=492 nm，Em=517 nm)。5-Carboxyfluorescein diacetate N-succinimidyl ester 可与细胞内分子共价结合，从而标记细胞。5-Carboxyfluorescein diacetate N-succinimidyl ester 可用于示踪淋巴细胞的迁移和增殖。
生物活性	5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation.
性状	Solid
体外研究(In Vitro)	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dilute the stock solution in PBS (to give a 50 μM solution). 2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly. 3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS. 4. Centrifuge cells 5 min at 300 ×g, 20 ℃. 5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS. 6. Centrifuge cells 5 min at 300 × g, 20 ℃, and remove the supernatant. Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Powder -20°C 3 years；In solvent -80°C 6 months
参考文献	[1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9.

溶解度数据	In Vitro: DMSO : 20.83 mg/mL (37.37 mM; ultrasonic and warming and heat to 60°C)配制储备液

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[1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9.

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

5-Carboxyfluorescein diacetate N-succinimidyl ester

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