β-Amyloid (35-42)
目录号: PL13288 纯度: ≥98%
CAS No. :183292-41-3
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中文名称
β-Amyloid (35-42)
英文名称
β-Amyloid (35-42)
英文别名
β-Amyloid (35-42)
Cas No.
183292-41-3
分子式
C33H60N8O9S
分子量
744.94
包装储存
Sealed storage, away from moisturePowder -80°C 2 years;-20°C 1 year
产品详情
β-Amyloid (35-42) 是 β 淀粉样蛋白的多肽片段,由 35-42 位氨基酸序列组成。
生物活性
β-Amyloid (35-42) is a peptide consisting of amino acid of 35 to 42 of beta amyloid protein.
性状
Solid
体外研究(In Vitro)
β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.
2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.
3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.
Met
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Sealed storage, away from moisturePowder -80°C 2 years;-20°C 1 year
SequenceShortening
MVGGVVIA
Sequence
Met-Val-Gly-Gly-Val-Val-Ile-Ala
参考文献
[1]. Hubin E, et, al. Two distinct β-sheet structures in Italian-mutant amyloid-beta fibrils: a potential link to different clinical phenotypes. Cell Mol Life Sci. 2015 Dec;72(24):4899-913.
溶解度数据
In Vitro: DMSO : 50 mg/mL (67.12 mM; Need ultrasonic)配制储备液
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1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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