Sealed storage, away from moisturePowder -80°C 2 years;-20°C 1 year
产品详情
Myelin Basic Protein (MHP4-14) TFA 是一种合成肽,包含髓鞘碱性蛋白的残基4-14,是一种非常有选择性的 PKC 底物 (Km=7 μM)。Myelin Basic Protein TFA 不被环腺苷酸依赖性蛋白激酶、酪蛋白激酶 I 和 II、Ca2+/钙调素依赖性蛋白激酶 II 或磷酸化酶激酶磷酸化,可常规用于测定粗组织提取物中低背景的蛋白激酶C。
生物活性
Myelin Basic Protein (MHP4-14) TFA, a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (K m =7 μM). Myelin Basic Protein TFA is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts.
性状
Solid
体外研究(In Vitro)
Once MBP4-14 is phosphorylated, it is not dephosphorylated by okadaic acid-sensitive phosphatases (protein phosphatases 1, 2A and 3) or other protein phosphatases such as calcineurin and/or PP 2C present in hippocampal homogenates. Therefore, MBP4-14 can be used for PKC assay in crude extracts of neural tissue. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Sealed storage, away from moisturePowder -80°C 2 years;-20°C 1 year
SequenceShortening
QKRPSQRSKYL
参考文献
[1]. Farrar YJ, et al. A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts. Biochem Biophys Res Commun. 1991;180(2):694-701. [2]. Yasuda I, et al. A synthetic peptide substrate for selective assay of protein kinase C. Biochem Biophys Res Commun. 1990;166(3):1220-1227.
溶解度数据
In Vitro: H2O : 100 mg/mL (66.46 mM; Need ultrasonic)配制储备液