Thrombin (MW 37kDa) (Synonyms: 凝血酶)
目录号: PL09424
CAS No. :9002-04-4
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中文名称
Thrombin (MW 37kDa)
中文别名
凝血酶;牛凝血酶;Thrombin 凝血酶;凝血酶 来源于牛血浆;凝血酶(牛血浆);凝血酶,BR;凝血酶标准品(JP);人血浆凝血酶2;凝血醇素;凝血酶,老鼠中提取;凝血酶,凝血因子;凝血酶,牛血浆;IIa 因子;凝血酶(Thrombin)牛血浆
英文名称
Thrombin (MW 37kDa)
英文别名
Thrombin;thrombin from bovine plasma;thrombin time;Thrombin, Bovine;Thrombin, Plasminogen-Free, Bovine;Thrombin, Human Plasma;Thrombin, Citrate-Free, HumanPlasma;thrombin from human plasma;THROMBIN FROM BOVINE PLASMA GR;New Zealan d Origin;Thrombin, from Bovine Plasma, Australia;thrombase
Cas No.
9002-04-4
分子式
C26H34N6O7S.C2H4O2
分子量
634.70
包装储存
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
产品详情
Thrombin (MW 37kDa) 是一种 Na+ 激活的胰蛋白酶样别构丝氨酸蛋白酶。Thrombin 能够利用凝血酶识别序列对融合蛋白进行位点特异性切割,并可用于消化 GST 标记的蛋白。
生物活性
Thrombin (MW 37kDa) is a Na-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
性状
Solid
体外研究(In Vitro)
p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h.
Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
参考文献
[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.
[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).
[3]. Geetha T, et al. p62 serves as a shuttling facto
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1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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