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Cas No.
9002-04-4
分子式
C26H34N6O7S.C2H4O2
分子量
634.70
包装储存
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Thrombin (MW 37kDa) is a Na-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
性状
Solid
体外研究(In Vitro)
p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h.Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
参考文献
[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).[3]. Geetha T, et al. p62 serves as a shuttling facto