Framycetin

(Synonyms: 新霉素B; Neomycin B; Fradiomycin B)
目录号: PL04761
Framycetin (Neomycin B) 是一种氨基糖苷类抗生素,是一种有效的 RNase P 裂解活性抑制剂,Ki 为 35 μM。Framycetin 竞争 RNase P RNA 中特定的二价金属离子结合位点。Framycetin 抑制锤头状核酶 (hammerhead ribozyme),Ki 值为 13.5 μM。Framycetin 是 5″-azido neomycin B 前体,与 miR-525 中的 Drosha 位点结合,可用于肝性脑病和肠致病性大肠杆菌感染。
CAS No. :119-04-0
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中文名称
Framycetin
中文别名
新霉素B六乙酸酯;4-(5-氯噻唑并[4,5-D]嘧啶-7-基)吗啉;硫酸弗兰西汀;新霉素 B;新霉素标液;新霉素乙硫酸(硫酸新霉素B);新霉素溶液;硫酸弗兰西汀(新霉素B);新霉素B
英文名称
Framycetin
英文别名
Neomycin B Hexaacetate;Neomycin B;NEOMYCIN B, EP STANDARD;...;3)-O-b-D-ribofuranosyl-(1®actilin;actiline;antibiotique;enterfram;Fradiomycin B;framygen;Neomycin solution;soframycin;soframycine;Framidal;Framycin;Francetin;Fraquinol;framycetin
Cas No.
119-04-0
分子式
C23H46N6O13
分子量
614.64
包装储存
Solution, -20°C, 2 years
详情描述
Framycetin (Neomycin B) 是一种氨基糖苷类抗生素,是一种有效的 RNase P 裂解活性抑制剂,Ki 为 35 μM。Framycetin 竞争 RNase P RNA 中特定的二价金属离子结合位点。Framycetin 抑制锤头状核酶 (hammerhead ribozyme),Ki 值为 13.5 μM。Framycetin 是 5″-azido neomycin B 前体,与 miR-525 中的 Drosha 位点结合,可用于肝性脑病和肠致病性大肠杆菌感染。
产品详情
Framycetin (Neomycin B) 是一种氨基糖苷类抗生素,是一种有效的 RNase P 裂解活性抑制剂,Ki 为 35 μM。Framycetin 竞争 RNase P RNA 中特定的二价金属离子结合位点。Framycetin 抑制锤头状核酶 (hammerhead ribozyme),Ki 值为 13.5 μM。Framycetin 是 5″-azido neomycin B 前体,与 miR-525 中的 Drosha 位点结合,可用于肝性脑病和肠致病性大肠杆菌感染。
生物活性
Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a K i of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a K i of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections.
性状
Liquid
IC50 & Target[1][2]
Aminoglycoside
体外研究(In Vitro)
The inhibition of RNase P RNA cleavage by Framycetin (Neomycin B; Fradiomycin B) is sensitive to pH and an increase in pH suppresses the inhibition in other systems.
Framycetin targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line.
Framycetin binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function.
Framycetin induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Solution, -20°C, 2 years
参考文献
[1]. N E Mikkelsen, et al. Inhibition of RNase P RNA Cleavage by Aminoglycosides. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6155-60.
[2]. Childs-Disney JL, et al. Small Molecule Targeting of a MicroRNA Associated with Hepatocellular Carcinoma. ACS Chem Biol. 2016 Feb 19;11(2):375-80.
[3]. Stampfl S, et al. Monova
溶解度数据
In Vitro: H2O : 100 mg/mL (162.70 mM; Need ultrasonic)DMSO : 50 mg/mL (81.35 mM; Need ultrasonic)In Vivo:
搜索质检报告(COA)
[1]. N E Mikkelsen, et al. Inhibition of RNase P RNA Cleavage by Aminoglycosides. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6155-60.
[2]. Childs-Disney JL, et al. Small Molecule Targeting of a MicroRNA Associated with Hepatocellular Carcinoma. ACS Chem Biol. 2016 Feb 19;11(2):375-80.
[3]. Stampfl S, et al. Monova

1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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