Fluorescein tyramide is a green fluorescent reagent (λ abs : 494 nm; λ em : 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications.
性状
Solid
体外研究(In Vitro)
HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years;In solvent -80°C 6 months
参考文献
[1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.[2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82.
[1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.[2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82.