Dabcyl-KTSAVLQSGFRKME-Edans TFA is a fluorogenic peptide. Dabcyl-KTSAVLQSGFRKME-Edans TFA is used as the substrate to measure the enzymatic activities of protease forms. Dabcyl-KTSAVLQSGFRKME-Edans TFA has the potential for study 2019-nCoV (COVID-19) infection.
性状
Solid
体外研究(In Vitro)
Recombinant 3CLpro is expressed in Pichia pastoris GS115 as a 42 kDa protein that displays a Km of 15 μM with Dabcyl-KTSAVLQSGFRKME-Edans TFA as substrate. The Dabcyl-KTSAVLQSGFRKME-Edans TFA fluorogenic peptide is used as the substrate and the enhanced fluorescence due to cleavage of this substrate catalyzed by the protease is monitored at 538 nm with an excitation wavelength of 355 nm using a fluorescence plate reader. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Sealed storage, away from moisture and light, under nitrogenPowder -80°C 2 years;-20°C 1 ye
[1]. Thi Thanh Hanh Nguyen, et al.Flavonoid-mediated Inhibition of SARS Coronavirus 3C-like Protease Expressed in Pichia Pastoris. Biotechnol Lett. 2012 May;34(5):831-8.[2]. Chih-Jung Kuo, et al.Engineering a Novel Endopeptidase Based on SARS 3CL(pro). Biotechniques. 2009 Dec;47(6):1029-32.[3]. Cheng-Wei Yang, et
溶解度数据
In Vitro: H2O : 6.67 mg/mL (3.04 mM; Need ultrasonic)配制储备液