SL327 抑制 MEK1 和 MEK2，IC₅₀ 分别为 180 nM 和 220 nM。

SL327 inhibits MEK1 and MEK2, with IC 50 values of 180 nM and 220 nM, respectively.

Solid

MEK1 180 nM (IC50) MEK2 220 nM (IC50

The specificity of SL327 for MEK is investigated. Kinase activity is assessed by measuring the incorporation of [32P]phosphate during phosphorylation of substrate peptides specific for each kinase. Although SL327 inhibits MEK with an IC50 of 0.27 μM, 10 μM SL327 has no significant effect on PKA, CaMKII, or PKC. has not independently confirmed the accuracy of these methods. They are for reference only.

SL327, which crosses the blood-brain barrier, is administered intraperitoneally at several concentrations to animals prior to cue and contextual fear conditioning. Administration of SL327 completely blocks contextual fear conditioning and significantly attenuates cue learning when measure 24 hr after training. Animals treated with SL327 exhibit significant attenuation of water maze learning; they take significantly longer to find a hidden platform compared with vehicle-treated controls and also fail to use a selective search strategy during subsequent probe trials in which the platform is removed. Mice are injected with various concentrations of SL327 (10, 30, 50 mg/kg i.p.), and 1 hr later their hippocampi are removed and assayed for activated MAPK. SL327 attenuates phosphorylated MAPK levels in a dose-dependent manner. Administration of 10, 30, or 50 mg/kg SL327 significantl

Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years；4°C 2 years

[1]. Cheng Y, et al. Current Development Status of MEK Inhibitors. Molecules. 2017 Sep 26;22(10). pii: E1551.
[2]. Selcher JC, et al. A necessity for MAP kinase activation in mammalian spatial learning. Learn Mem. 1999 Sep-Oct;6(5):478-90.

In Vitro: DMSO : 68 mg/mL (202.77 mM; Need ultrasonic)Ethanol : 0.1 mg/mL (0.30 mM; Need ultrasonic and warming)配制储备液