ML346是 Hsp70 和 HSF-1 活性的激活剂，针对 Hsp70 的 EC₅₀ 为 4.6 μM。ML346 恢复构象疾病模型中的蛋白质折叠，而没有明显的细胞毒性或缺乏特异性。ML346 诱导了热休克反应 (HSR) 的基因和蛋白质效应子的特异性增加，包括伴侣蛋白如 Hsp70，Hsp40 和 Hsp27。

ML346 is an activator of Hsp70 expression and HSF-1 activity, with an EC 50 of 4.6 μM for Hsp70. ML346 restores protein folding in conformational disease models, without significant cytotoxicity or lack of specificity. ML346 induces specific increases in genes and protein effectors of the heat shock response (HSR), including chaperones such as Hsp70, Hsp40, and Hsp27.

Solid

HSP70 4.6 μM (EC50, HeLa cells)

ML346 is an activator of Hsp70, with an EC50 of 4600 nM in HeLa cells. ML346 (10 μM) restores proteostasis, restores CFTR-mediated iodide conductance, and enhances the correct folding of proteins expressed in two different cellular compartments. ML346 (Compound F1) induces multiple responses and strongly induces Hsp70, the oxidative stress response genes (HO1 and GCLM), and a 2.5-fold upregulation of BiP in WT MEF cells. ML346 (0.5-25 μM) exhibits cytoprotective effects in cells after a 35 min severe heat shock, and also causes a two-fold protection from H2O2-induced apoptosis. has not independently confirmed the accuracy of these methods. They are for reference only.

ML346 suppress the aggregation of polyQ35 in a C. elegans model, suggesting the probe has efficacy in modifying protein aggregation and associated toxicity. has not independently confirmed the accuracy of these methods. They are for reference only.

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Powder -20°C 3 years；4°C 2 years

[1]. Calamini B, et al. ML346: A Novel Modulator of Proteostasis for Protein Conformational Diseases.Probe Reports from the NIH Molecular Libraries Program. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. 2012 Dec 17.
[2]. Calamini B, et al. Small-molecule proteostasis regulators for protein conformational diseases. Nat Chem Biol. 2011 Dec 25;8(2):185-96.

In Vitro: DMSO : 12.5 mg/mL (45.91 mM; Need ultrasonic)配制储备液