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4-Methylumbelliferyl-β-D-glucuronide hydrate

(Synonyms: MUG)
目录号: PL14165 纯度: ≥99%
4-Methylumbelliferyl-β-D-glucuronide hydrate 是一种荧光底物 (λex=362 nm,λem=445 nm)。4-Methylumbelliferyl-β-D-glucuronide hydrate 在检测 β-葡萄糖醛酸酶的活性和大肠杆菌数中有潜在应用。
CAS No. :881005-91-0
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中文名称
4-Methylumbelliferyl-β-D-glucuronide hydrate
中文别名
4-甲基-2-氧代-2H-1-苯并吡喃-7-基-beta-D-葡糖苷酸;4-甲基伞形酮-β-D-葡糖苷酸二水合物;4-甲基伞形酮-β-D-葡萄糖糖苷;6-氯-3,4-二氢-4-甲基-3-氧代-2H-1,4-苯并恶嗪-8-羧酸甲酯;4-甲基伞形酮-Β-D-葡糖苷酸二水合物
英文名称
4-Methylumbelliferyl-β-D-glucuronide hydrate
英文别名
b-D-Glucopyranosiduronic acid,4-methyl-2-oxo-2H-1-benzopyran-7-yl, hydrate (9CI);4-Methylumbelliferyl beta-D-Glucuronide Hydrate;(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid,hydrate;4'-METHYLUMBELLIFERYL-ß-D-GLUCURONIDE (MUG);MUG;4-Methylumbelliferyl-β-D-glucopyranosiduronic acid;4-Methylumbelliferyl-β-D-glucuronide hydrate;4-Methylumbelliferyl-;A-D-glucuronide hydrate;4-Methylumbelliferyl-beta-D-glucuronide hydrate;A-D-glucuronide H2O;C16H16O9.H2O;KM2573;W0272;4-Methylumbelliferyl-beta-D-glucuronide hydrate, BioXtra;4-Methyl-2-oxo-2H-1-benzopyran-7-yl beta-D-glucopyranosiduronic acid--water (1/1);4-Methylumbelliferyl-beta-D-glucuronide hydrate, suitable for fluorescence, >=9
Cas No.
881005-91-0
分子式
C16H18O10
分子量
370.31
包装储存
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
详情描述
4-Methylumbelliferyl-β-D-glucuronide hydrate 是一种荧光底物 (λex=362 nm,λem=445 nm)。4-Methylumbelliferyl-β-D-glucuronide hydrate 在检测 β-葡萄糖醛酸酶的活性和大肠杆菌数中有潜在应用。
产品详情
4-Methylumbelliferyl-β-D-glucuronide hydrate 是一种荧光底物 (λex=362 nm,λem=445 nm)。4-Methylumbelliferyl-β-D-glucuronide hydrate 在检测 β-葡萄糖醛酸酶的活性和大肠杆菌数中有潜在应用。
生物活性
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ ex =362 nm, λ em =445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
性状
Solid
体外研究(In Vitro)
4-Methylumbelliferyl-β-D-glucuronide hydrate (156 μM-5 mM;30 min) 在 37℃ 下释放 4-甲基伞形酮,用于酶促反应。
4-Methylumbelliferyl-β-D-glucuronide hydrate (50 μg/mL;24 h) 在 TSA 培养基中用于检测大肠杆菌数。
has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
参考文献
[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.
[2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4.
溶解度数据
In Vitro: DMSO : 150 mg/mL (405.07 mM; Need ultrasonic)H2O : 5.2 mg/mL (14.04 mM; Need ultrasonic and warming)配制储备液
搜索质检报告(COA)
[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.
[2]. Sperker B, et al. High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates. J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):181-4.

1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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