HBC514 本身是一种非荧光的 HBC 模拟物，但在与 Pepper RNA 适配体形成紧密复合物后可以发出强烈的绿色荧光，用于 RNA 标记。在活的 Pepper 标记的细胞中，HBC514-Pepper complex 可以使 RNAs 可视化，并且可以通过简单的洗涤和染色灵活地改变荧光。

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

Solid

IC50: RNA fluorescent dye

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells.
‘Pepper’ has reduced Mg sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).
has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years；4°C 2 years

[1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.Nat Biotechnol. 2019 Nov;37(11):1287-1293.

In Vitro: DMSO : 25 mg/mL (75.66 mM; Need ultrasonic)配制储备液