Glycine,N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-[(5-oxo-2-thioxo-4-imidazolidinylidene)methyl]-6-benzofuranyl]-,(acetyloxy)methyl ester;acetyloxymethyl 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[[6-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-2-[(E)-(5-oxo-2-sulfanylideneimidazolidin-4-ylidene)methyl]-1-benzofuran-5-yl]oxy]ethoxy]-4-;Fura 2-TH,;Fura 2-TH, AM(Known as Fura Red™, TM of Molecular Probes);Glycine,N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethy...;Glycine,N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy;Glycine,N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-[(5-oxo-2-thioxo-4-imidazolidinylidene)methyl]-6-benzofuranyl]-,(acetyl;(acetyloxy)methyl [{2-[(acetyloxy)methoxy]-2-oxoethyl}(5-{2-[2-(bis{2-[(acetyloxy)methoxy]-2-oxoethyl}amino)-5-methylphenoxy]ethoxy}-2-[(E)-(5-oxo-2-thioxoimidazolidin-4-ylidene)methyl]-1-benzofuran-6-yl)amino];Fura 2-TH, AM Fura red;Glycine, N-(2-((acetyloxy)methoxy)-2-oxoethyl)-N-(5-(2-(2-(bis(2-((acetyloxy)methoxy)-2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-((5-oxo-2-thioxo-4-imidazolidinylidene)methyl)-6-benzofuranyl)-, (acetyloxy)methyl ester;Fura 2-TH, AM (Known as Fura Red(TM), TM of Molecular Probes)
Cas No.
149732-62-7
分子式
C41H44N4O20S
分子量
944.87
包装储存
Please store the product under the recommended conditions in the Certificate of Analysis.
详情描述
Fura Red 是一种 Ca2+ 敏感型荧光染料,荧光随 [Ca2+] 浓度升高而降低。
产品详情
Fura Red 是一种 Ca2+ 敏感型荧光染料,荧光随 [Ca2+] 浓度升高而降低。
生物活性
Fura Red is a Ca-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca].
体外研究(In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Incubate the cells according to your normal protocol. 2. Suspend cells at 1x10 cells/mL in 37°C Hanks Balanced Salt Solution (HBSS) with 1 μM Fura Red, AM unless specified otherwise and 0.01% Pluronic F127, and incubate in a 37°C water bath for 30 minutes. 3. Wash cells in HEPES Buffered Saline Solution (HBSS with 1 mM CaCl2, 0.5 mM MgCl2, 0.1% BSA, 10 mM HEPES) and resuspend to 1x10 cells/mL in HEPES Buffered Saline Solution with viability dye, SYTOX Green. 4. Equilibrate cells for ≥10 minutes in a 37°C water bath. 5. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. Ratiometric analysis of Fura Red is measured
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Floto RA, et al. IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes. Cell Calcium. 1995 Nov;18(5):377-89.[2]. Wendt ER, et al. Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets. PLoS One. 2015 Apr 2;10(3):e0119532.
[1]. Floto RA, et al. IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes. Cell Calcium. 1995 Nov;18(5):377-89.[2]. Wendt ER, et al. Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets. PLoS One. 2015 Apr 2;10(3):e0119532.