Nile blue;Nile Blue A;C.I. 51180;[9-(diethylamino)benzo[a]phenoxazin-5-ylidene]azanium,sulfate;Nile Blue Hydrogensulfate;5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1);9-(diethylamino)-5-imino-5h-;Basic Blue 12,Nile blue sulfate;Basic Blue-?12;CI 51180;EINECS 222-832-5;Nile Blue A sulfate;Nile Blue A, certified, pure;Nile Blue A, pure, certified;Nile Blue A,certified;Nile blue, sulfate;Basic Blue 12;Benzo[.alpha.] phenoxazin-7-ium, 5-amino-9-(diethylamino)-, sulfate;5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iu sulfate;Bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium] sulphate;Nile blue sulfate;Benzo[a]phenoxazin-7-ium, 5-amino-9-(diethylamino)-, sulfate (2:1);5H-Benzo(a)phenazoxazine, 9-(diethylamino)-5-imino-, sulfate (2:1);Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) sulphate;BENZO(a)PHENOXAZIN-7-IUM, 5-AMINO-9-(DIETHYLAMINO)-, SULFATE (2:1);2C20H20N3O
Cas No.
3625-57-8
分子式
C40H40N6O6S
分子量
732.85
包装储存
4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
详情描述
Nile Blue A (Nile blue sulfate) 可用于区分黑色素和脂褐素。它也可用于脂肪染色,以及制备电流葡萄糖传感器。
产品详情
Nile Blue A (Nile blue sulfate) 可用于区分黑色素和脂褐素。它也可用于脂肪染色,以及制备电流葡萄糖传感器。
生物活性
Nile Blue A (Nile blue sulfate) is used to differentiate melanins and lipofuscins. It is also useful for staining fats and preparation of an amperometric glucose sensor.
性状
Solid
体外研究(In Vitro)
Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
参考文献
[1]. Ostle AG, et al. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol. 1982 Jul;44(1):238-41.[2]. Betscheider D, et al. Nile blue A for staining Escherichia coli in flow cytometer experiments. Anal Biochem. 2009 Jan 1;384(1):194-6.[3]. Mishra SS, et al. Spectroscopic invest
[1]. Ostle AG, et al. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol. 1982 Jul;44(1):238-41.[2]. Betscheider D, et al. Nile blue A for staining Escherichia coli in flow cytometer experiments. Anal Biochem. 2009 Jan 1;384(1):194-6.[3]. Mishra SS, et al. Spectroscopic invest