Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λ ex =485 nm, λ em =535 nm).
性状
Solid
体外研究(In Vitro)
The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20°C, protect from light 该产品在溶液状态不稳定,建议您现用现配,即刻使用。
Emission(Em)
Em 495-570 nm Green
Excitation(Ex)
Ex 450-495 nm Blue
参考文献
[1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.
溶解度数据
In Vitro: DMSO : 5.56 mg/mL (8.47 mM; ultrasonic and warming and heat to 60°C)配制储备液