Coppersensor-1 (CS1) 是一种膜透性荧光染料。Coppersensor-1 对 Cu⁺ 具有亲和力，其亲和力在皮摩尔范围，对竞争细胞金属离子具有很高的选择性。Coppersensor-1 作为探针，可以选择性、灵敏地检测包括活细胞在内的生物样品中的铜 (I) 离子 (Cu⁺)。Coppersensor-1 可用于癌症、心血管疾病、神经系统疾病等严重疾病的成像研究。

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.

Oil

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Preparation of reagent stock solutions for imaging:
1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.
2. Prepare a 10 mM stock solution of CuCl2 (MW 134 g/moL) by dissolving 1.34 mg of solid CuCl2 per milliliter of Millipore water solvent.
B. CS1 labeling of live cells:
1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.

Room temperature in continental US; may vary elsewhere.

Pure form -20°C 3 years；4°C 2 years

[1]. Evan W Miller, et al. Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nat Protoc. 2006;1(2):824-7.