PETCM 是 caspase-3 的激活剂，以细胞色素 c (cyto c) 依赖的方式起作用。PETCM 可促进 Apaf-1 低聚化，可诱导 HeLa 细胞的凋亡 (apoptosis)。

PETCM is an activator of caspase-3 and acts as an cytochrome c (cyto c)-dependent manner. PETCM promotes Apaf-1 oligomerization and induces cell apoptosis in HeLa cells.

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Caspase 3

PETCM (0.1-0.5 mM) stimulates caspase-3 activity (DEVD activity) of HeLa S-100 in a dose-dependent manner.And 0.2 mM PETCM is more efficient in activating caspase-3 than 1 mM dATP.
PETCM (0.2 mM; 1 hour) drives apoptosome formation. Apaf-1 in a normal HeLa cell S-100 fraction is in an inactive monomeric form. After 1 mM dATP, most of the Apaf-1 shifted to a size of 1 million daltons. After the S-100 fraction with 0.2 mM PETCM, Apaf-1 exhibits a similar shift. And the efficiency of apoptosome formation was better with 0.2 mM PETCM.
PETCM (0.2 mM; 1 hour) can antagonize the inhibitory activity of ProT reduced caspase-3 activation in in HeLa cells.
has not independently confirmed the accuracy of these methods. They are for reference only.

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[1]. Nguyen JT, et al. Direct activation of the apoptosis machinery as a mechanism to target cancer cells.Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8.
[2]. .Jiang X, Kim HE, Shu H, et al. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science, 2003, 299(5604): 223-226.

In Vitro: DMSO : 100 mg/mL (415.78 mM; ultrasonic and heat to 40°C)配制储备液