Phloretin|60-82-2|(根皮素; NSC 407292; RJC 02792)-陌孚医药/Medlife

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Phloretin (Synonyms: 根皮素; NSC 407292; RJC 02792)

目录号: PC10640 纯度: ≥98%

CAS No. :60-82-2

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PC10640-500mg	500mg	¥637.00	请登录
PC10640-10mM (in 1mL DMSO)	10mM (in 1mL DMSO)	¥637.00	请登录

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中文名称

Phloretin

中文别名

根皮素;2',4',6'-三羟基-3-(4-羟基苯基)苯丙酮;三羟苯酚丙酮;根皮酸苯三酚一酯;Phloretin 根皮素;根皮苷;根皮素标准品;根皮素(标准品);根皮素,Phloretin,植物提取物,标准品,对照品;根皮素Phloretin;三羟苯酚丙酮对照品;3-(4-羟苯基)-1-(2,4,6-三羟基苯基)-1-丙酮;根皮素(根皮酸苯三酚一酯,三羟苯酚丙酮);根皮素, 三羟苯酚丙酮

英文名称

Phloretin

英文别名

Phloretin;2',4',6',4-TETRAHYDROXYDIHYDROCHALCONE;2',4',6'-TRIHYDROXY-3-(4-HYDROXYPHENYL)-1-PROPIOPHENONE;2',4',6'-TRIHYDROXY-3-(4-HYDROXYPHENYL)-PROPIOPHENONE;2',4',6'-TRIHYDROXY-3-P-HYDROXYPHENYLPROPIOPHENONE;2,4,6-TRIHYDROXY-BETA-(4-HYDROXYPHENYL)PROPIOPHENONE;3-[4-HYDROXYPHENYL]-1-[2,4,6-TRIHYDROXYPHENYL]-1-PROPANONE;4,2',4',6'-TETRAHYDROXYDIHYDROCHALCONE;BETA-(4-HYDROXYPHENYL)-2,4,6-TRIHYDROXY-PROPIOPHENONE;PHLORETIN (NATURAL);3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one;2,4,6-Trihydroxy-B-(4-Hydroxyphenyl)Propiophenone;β-(4-hydroxyphenyl)-2,4,6-trihydroxypropiophenone;PHLORETIN(RG);1-Propanone, 3-(4-hydroxyphenyl)-1-(2,4,6-;Dihydronaringenin;Floretin;NSC 407292;Phloretol;RJC 02792;UNII-S5J5OE47MK;2′,4′,6′-Trihydroxy-3-(4-hydroxyphenyl)propiophenone;3-(4-Hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)-1-propanone;2',4',6'-Trihydroxy-3-(4-hydroxyphenyl)propiophenone;[ "" ];1-Propanone, 3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)-;beta-(p-Hydroxyphenyl)phloropropiophenone;2',4',6'-Trihydroxy-3-(p-hydroxyphenyl)propiophenone;NSC407292;S5J5OE47MK;beta-(p-Hydroxyphenyl)-2,4,6-trihydrox

Cas No.

60-82-2

分子式

C₁₅H₁₄O₅

分子量

274.27

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

生物活性

Phloretin (NSC 407292; RJC 02792) is a flavonoid extracted from Malus pumila Mill., has anti-inflammatory activities. Phloridzin is a specific, competitive and orally active inhibitor of sodium/glucose cotransporters in the intestine (SGLT1) and kidney (SGLT2). Phloretin inhibits Yeast-made GLUT1 as well as Human erythrocyte GLUT1 with IC₅₀values of 49 μM and 61 μM, respectively.Phloretin has the potential for the treatment of rheumatoid arthritis (RA) and allergic airway inflammation.

性状

Solid

IC50 & Target[1][2]

Human Endogenous Metabolite

Microbial Metabolite

GLUT1

GLUT2

体外研究(In Vitro)

Phloretin induced obvious cytotoxicity against BEL-7402 cells with IC₅₀ of 89.23 μM.
Phloretin (40-160 μM; 24 hours) induces BEL-7402 cell apoptosis though the mitochondrial pathway, the cells exposed to phloretin exhibits nuclear chromatin condensation and increased fluorescence intensity. The caspase-9 reaches the peak level at 12 h, and leak levels of caspase-6 and caspase-3 activities occurrs 18 and 24 h after the exposure, respectively.
Phloretin (0-100 μM; 24 hours) has effects on transcription factors of adipogenesis in differentiated 3T3-L1 adipocytes, decreases PPAR-γ, C/EBPα and C/EBPβ as a dose-dependent manner.
Phloretin (0-100 μM; 24 hours) has effects on the AMPK pathway in differentiated 3T3-L1 adipocytes, increases the phosphorylation of substrate ACC-1, AMPK in PT-treated cells.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis

Cell Line:	BEL-7402 cell
Concentration:	40-160 μM
Incubation Time:	24 hours
Result:	Induced cell apoptosis and activated caspase 3, 6 and 9.

Western Blot Analysis

Cell Line:	3T3-L1 adipocytes
Concentration:	0 μM, 3 μM, 10 μM, 30 μM, 100 μM
Incubation Time:	24 hours
Result:	Inhibited PPAR-γ, C/EBPα and C/EBPβ expression.

Western Blot Analysis

Cell Line:	3T3-L1 adipocytes
Concentration:	0 μM, 3 μM, 10 μM, 30 μM, 100 μM
Incubation Time:	24 hours
Result:	Upregulated the expression of p-ACC-1, p-AMPK, p-AMPKα and β .

体内研究(In Vivo)

Phloretin (oral administration; 50 or 100?mg/kg; once daily) decreases oxidative stress and diminished levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in paw tissue, reduces productivity of anti-collagen antibodies in serum.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Collagen-Induced Arthritis (CIA) Mice
Dosage:	50 or 100?mg/kg
Administration:	Oral adminstration
Result:	Showed mitigation of clinical symptoms of RA in addition to reduced inflammation of hind-limbs compared to the control group.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

结构分类

来源

参考文献

[1]. Kasahara T, Kasahara M. Expression of the rat GLUT1 glucose transporter in the yeast Saccharomyces cerevisiae. Biochem J. 1996 Apr 1;315 ( Pt 1):177-82. [Information]

[2]. Luo H, et al. Phloretin induces apoptosis of BEL-7402 cells 体外研究. Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jul;28(7):1249-51. [Information]

[3]. Huang WC, et al. Phloretin and phlorizin promote lipolysis and inhibit inflammation in mouse 3T3-L1 cells and in macrophage-adipocyte co-cultures.Mol Nutr Food Res. 2013 Oct;57(10):1803-13. [Information]

[4]. Wang SP, et al. Potent Antiarthritic Properties of Phloretin in Murine Collagen-Induced Arthritis.Evid Based Complement Alternat Med. 2016;2016:9831263. [Information]

溶解度数据

体外研究:

DMSO : 50 mg/mL (182.30 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (ultrasonic) (insoluble)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6460 mL	18.2302 mL	36.4604 mL
5 mM	0.7292 mL	3.6460 mL	7.2921 mL
10 mM	0.3646 mL	1.8230 mL	3.6460 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.12 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液
2.

建议依照次序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.12 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

建议依照次序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.12 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

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产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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