L-Azidonorleucine hydrochloride 是一种非天然氨基酸，是 Methionine 的替代物。L-Azidonorleucine hydrochloride 可用于标记哺乳动物细胞蛋白，并鉴定多种甲硫酰基-tRNA 合成酶 (MetRS) 突变体。

L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants .

Solid

CHO cells transfected with pMaRSC and incubated with L-Azidonorleucine (Anl; 1.5 mM; 6 h) are fixed, permeabilized, and treated with alkyne-TAMRA. The TAMRA fluorescence exclusively in the mCherry-expressing cells is observed, consistent with cell-selective incorporation of Anl into cellular proteins. has not independently confirmed the accuracy of these methods. They are for reference only.

When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired. has not independently confirmed the accurac

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moistur In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

[1]. Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81.
[2]. Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575.

In Vitro: H₂O : 125 mg/mL (599.09 mM; Need ultrasonic)配制储备液