Poly-D-lysine hydrobromide (MW 70000-150000) is a synthetic polymeric substrate and is one of the most widely used substrate in neural cell culture. Poly-D-lysine hydrobromide (MW 70000-150000) removes proteinase K-resistant PrP from prion-infected SN56 neuroblastoma cells without affecting PrP.
性状
Solid
体外研究(In Vitro)
Poly-D-lysine is immunogenic in rabbits only when injected at low doses.The approximate concentration of Poly-D-lysine (PDL) needed to lower the amount of PrPres by 50% corresponds to ~0.8 μg/mL or 50 nM. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years;In solvent -80°C 6 months
参考文献
[1]. HansVermeersch, et al. Immunogenicity of poly-D-lysine, a potential polymeric drug carrier. Journal of Controlled Release. Volume 32, Issue 3, 1 December 1994, Pages 225-229. [2]. Zhou Xu, et al. Mechanistic insights into cellular alteration of prion by poly-D-lysine: the role of H2H3 domain. FASEB J. 2011 Oct;25(10):3426-35.
[1]. HansVermeersch, et al. Immunogenicity of poly-D-lysine, a potential polymeric drug carrier. Journal of Controlled Release. Volume 32, Issue 3, 1 December 1994, Pages 225-229. [2]. Zhou Xu, et al. Mechanistic insights into cellular alteration of prion by poly-D-lysine: the role of H2H3 domain. FASEB J. 2011 Oct;25(10):3426-35.