Biotin-16- dUTP (Biotin-16-deoxyuridine-5-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP trisodium can be used to produce biotinylated DNA probes in a variety of assay applications.
性状
Liquid
体外研究(In Vitro)
Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis.Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin. has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Solution, -20°C, 2 years
参考文献
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4.[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11.