Biotin-16-dUTP trisodium (Synonyms: Biotin-16-deoxyuridine-5'-triphosphate trisodium)
目录号: PL05613 纯度: ≥97%
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PL05613-25μg(1mM*24.66μLinWater) 25μg(1mM*24.66μLinWater) ¥3616.00 请登录
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中文名称
Biotin-16-dUTP trisodium
英文名称
Biotin-16-dUTP trisodium
包装储存
Solution, -20°C, 2 years
产品详情
Biotin-16- dUTP (Biotin-16-deoxyuridine-5'-triphosphate) trisodium 通过酶促掺入 DNA/cDNA 中,可替代其天然对应物 dTTP。Biotin-16- dUTP trisodium 可用于在各种检测应用中生产生物素化 DNA 探针。
生物活性
Biotin-16- dUTP (Biotin-16-deoxyuridine-5-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP trisodium can be used to produce biotinylated DNA probes in a variety of assay applications.
性状
Liquid
体外研究(In Vitro)
Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis.
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin.
has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Solution, -20°C, 2 years
参考文献
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4.
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11.
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1:一般建议:溶解度为Medlife测试数据,可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异,仅做参考,具体以实验方案为准。

2:储存条件:粉末-20°C一般情况可以保存3年,溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异,请细致阅读产品信息,并辅助参考相关文献描述。

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