脑脊液的替代物

ACSF通常用作CSF的替代物，可在脑片灌注过程中保护中间神经元 [1].

ACSF (人工脑脊液)可减少脑水肿的发生率，并进一步抑制脑细胞疾病。在神经外科领域，如颅内手术，ACSF常被用作冲洗液或灌洗液，或者当脑脊液流失时用作补充液。在科研中ACSF常用来维持pH平衡和维持组织氧输送[2，3]。

在体外急性大脑切片模型中研究神经功能的基本特征时，ACSF常用来维持氧的供给。在新皮层小鼠切片中，carbogenated ACSF处理可保持pH平衡和维持氧输送，而no-magnesium ACSF治疗的切片中出现癫痫样事件[1]。在保护神经元的靶向药物研究中，通常用ACSF处理小鼠作为对照来评估药效[3]。

也有研究指出在大鼠脑切片模型中，经ACSF（人CSF的替代物）处理可有力地促进CA1、CA3区和5层锥体神经元的自发放电[4]。

参考文献：

[1] Voss, L. J., S.A. George, and J.W. Sleigh, Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. J Neurosci Methods, 2012. 204(2): p. 273-5.

[2] Liu, D.  and F. Bao, Hydrogen peroxide administered into the rat spinal cord at the level elevated by contusion spinal cord injury oxidizes proteins, DNA and membrane phospholipids, and induces cell death: Attenuation by a metalloporphyrin. Neuroscience, 2015. 285: p. 81-96.

[3] Gruber, R. C., et al., Targeted GAS6 delivery to the CNS protects axons from damage during experimental autoimmune encephalomyelitis. J Neurosci, 2014. 34(49): p. 16320-35.

[4] Bjorefeldt, A. , et al., Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice. J Physiol, 2015. 593(1): p. 231-43.