FITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu-sensitive fluorescence dye.

Solid

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Room temperature or refrigerated transportation.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• Description: FITC can be used as a fluorescence probe for nanocomposites with green fluorescence.
  Method: For nanocomposites linking.
  1. Add nanocomposite to EDC and NHS (molar ratio: 1:5:5), and the pH is adjusted to activate the carboxyl groups.
  2. Dissolve FITC in dimethyl sulfoxideab and add to the above mixture and shaked in the dark overnight.
  3. The final FITC-labeled nanocomposite is obtained by lyophilization.
  4. Use a probe-based confocal laser endoscopy (Cellvizio, Mauna Kea Technologies, France) for determination.
• Description: FITC can be used as a fluorescence probe for nanocomposites with green fluorescence.
  Method: For nanocomposites linking.
  1. Suspend nanocomposites (20 mg) in PBS (20 mL) with FITC (1 mg) and put the mixture stirred overnight in the dark.
  2. Wash nanocomposites for five times with PBS to remove excess FITC.
  3. Testing cells are seeded into a 6-well microplate at a density of 10/well and cultured overnight at 37°C in 5% CO₂.
  4. Change the culture medium with as-prepared medium containing nanocomposite-FITC. After coincubation for 1, 2, 4, and 8 h, testing cells are rinsed three times with PBS.
  5. Use a confocal laser scanning microscopy ( FV1000 Olympus, Tokyo, Japan) for image.
• Description: FITC can be used as a fluorescence probe for linking to molecule glue with green fluorescence.
• Description: FITC can be used as a fluorescence probe for labeling lipophilic phytotoxin with green fluorescence.
• Description: FITC can be used as a fluorescence probe for labeling laccase with green fluorescence.
  Method: For laccase labeling.
  1. FITC (1 mg/mL) solution is prepared with dimethyl sulfoxide.
  2. Dropwise add FITC into laccase solution (5 mg/mL), and stir the mixed solution at 4°C for 4 h.
  3. Add NH₄Cl aqueous solution (2 mL, 50 mM) into the mixed solution to stop the reaction.
  4. Dialyze the solution in phosphate buffer (50 mM, pH 7) for 48 h at 4°C to remove excess FITC.
  5. Use a confocal laser scanning microscopy (Leica SP8 STED 3X) for image.

• [1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.  [Information]

  [2]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.  [Information]

  [3]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71  [Information]

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。