PD180970 (0.5 μM; 24-96 hours) treatment causes cell death K562 cells.
PD180970 (0.5 μM; 24-48 hours) treatment induces apoptosis of K562 cells. The result shows increase in annexin V-PI double-positive cells.
PD180970 inhibits tyrosine phosphorylation of p210Bcr-Abl, Gab2, and CrkL in K562 cells with IC50 values of 170 nM, 80 nM, and 80 nM, respectively. 体外研究, PD180970 potently inhibits autophosphorylation of p210 (IC50 of 5 nM) and the kinase activity of purified recombinant Abl tyrosine kinase (IC50 of 2.2 nM).
The blocking Bcr-Abl kinase activity using PD180970 in the human K562 CML cell line resulted in inhibition of Stat5 DNA-binding activity with an IC50 of 5 nM.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay
Cell Line: |
K562 cells
|
Concentration: |
0.5 μM |
Incubation Time: |
24 hours, 48 hours, 72 hours, 96 hours |
Result: |
Resulted in cell death. |
Apoptosis Analysis
Cell Line: |
K562 cells
|
Concentration: |
0.5 μM |
Incubation Time: |
24 hours, 48 hours |
Result: |
Increased annexin V-positive/PI-negative cells. |