荧光钙探针 Fura 2-AM;钙离子荧光探针 Fura 2-AM;AM荧光探针;钙离子荧光探针;钙荧光探针FURA-2, AM;FURA-2/AM荧光探针;FURA 2-AM SPECIAL PACKAGING
英文名称
Fura 2-AM
英文别名
Fura 2-AM;Fura-2 pentakis(acetoxymethyl) ester;2-(6-(Bis(2-((acetyloxy)methoxy)-2-oxoethyl)amino)-5-(2-(2-(bis(2-((acetylxoy)methoxy)-2-oxoethyl)amino)-5-methylphenoxy)ethoxy)-2-benzofuranyl)-5-oxazolecarboxylic acid (acetyloxy)methyl ester;Fura-2, AM;acetyloxymethyl 2-[5-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-4-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate;Fura 2-AM Special Packaging;Fura-2-Acetoxymethyl ester;FURA 2;Fura 2am;Fura 2-AM 1mM;Fura-2(AM);l DMSO Solution;Fura?-AM;Fura 2 Am Ester;Fura-2 acetoxymethyl;fura 2-am yellow powder;Fura 2 acetoxymethyl ester;AM in Solution;AM, FOR FLUORESCENCE;FURA-2;FURA 2/AM;FURA 2/AM in Solution;FURA-2/AM, FOR FLUORESCENCE;FURA2/AM *1SET = 10x100UG**;Fura-2, AM *CAS 108964-32-5*;Fura-2 AM
Cas No.
108964-32-5
分子式
C44H47N3O24
分子量
1001.85
包装储存
-20°C, protect from light
*该产品在溶液状态不稳定,建议您现用现配,即刻使用。
生物活性
Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca indicator.
性状
Liquid
体外研究(In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1. First, prepare the 1 mM Fura-2 AM stock by adding 50 μL of DMSO to a 50 μg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 μL of Fura-2 AM stock to generate a 1μM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6. Mount the coverslip on the imaging chamber.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.