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HEPES

目录号: PC19372 纯度: ≥99.5%

CAS No. :7365-45-9

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中文名称

HEPES

中文别名

N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸;2-[4-(羟乙基)-1-哌嗪基]乙磺酸;N-(2-羟乙基)哌嗪-N'-乙烷磺酸;4-羟乙基哌嗪乙磺酸;4-(2-羟乙基)-1-哌嗪乙磺酸;N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES);羟乙基哌嗪乙磺酸;2-[4-(2-羟乙基)-1-哌嗪乙烷]磺酸;N-2-羟乙基哌嗪-N'-2-乙磺酸;2-[4-(2-羟乙基)-1-哌嗪基]乙磺酸[生物研究用Good's缓冲液中的成分];2-[4-(2-羟乙基)-1-哌嗪基]乙磺酸;4-(2-羟乙基)-1-哌嗪乙烷磺酸;4-(2-羟乙基)哌嗪-1-乙磺酸;4-羟乙基哌嗪乙磺酸,BR;4-羟乙基哌嗪乙磺酸,进分;HEPES N-2-羟乙基哌嗪-N’-2-乙基磺酸;HEPES缓冲盐;HEPES缓冲液;HEPES羟乙基哌嗪乙磺酸;N-(2-羟乙基)哌嗪-N'-(2-乙磺酸);N-(2-羟乙基)哌嗪-N-(2-乙磺酸);N-(2-羟乙基)哌嗪-N''-2-乙烷磺酸;N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸,HEPES;N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸原药;N-2-羟乙基哌嗪-N''-2-乙磺酸;N-2-羟乙基哌嗪-N-2-乙磺酸钠盐;缓冲剂N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸;4-(2-羟乙基)-1-哌嗪基]乙磺酸;4-羟乙;N-（2-羟乙基）哌嗪-N -乙烷磺酸;N-(2-羟乙基)哌嗪-N’-2-乙烷磺酸;N-(2-羟乙基)哌嗪-N-2-乙烷磺酸;羟乙基哌嗪乙磺酸,4-(2-羟乙基)-1-哌嗪乙磺酸,N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸;羟乙基哌嗪乙硫磺酸;生物技术级;N-(2-羟乙基)哌嗪-N′-(2-乙磺酸);羟乙基哌嗪乙磺酸(HEPES);羟乙基哌嗪乙烷磺酸

英文名称

HEPES

英文别名

2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid;HEPES;2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid;hepes free acid molecular biology*reagent;4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid;hepes buffer (1M) aseptically filled*endotoxin te;HEPES, Free Acid, ULTROL Grade;HEPES, 1M solution;HEPES, Free Acid, MB Grade (1.12041);HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);HEPES, Tissue Culture Tested N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested;HEPES, Molecular Biology Grade N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade;HEPES buffer Solution;HEPES free acid;4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid;2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research];2-[4-(2-Hydroxyethyl)piperazin-4-ium-1-yl]ethanesulfonate;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID, MOLECULAR BIOLOGY GRADE;2Na;EIT hydrobromide;GOOD'S BUFFER;HEPES BUFFER PH 8.0;hepes solution;HEPES.1;N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid;4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acidacid);Hydroxyethylpiperazine Ethane Sulfonic Acid;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-;Monosodium Salt, HEPES;4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID;4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acid;N-2-Hydroxyethylpiperazine-N'-ethanesulfonate;N-2

Cas No.

7365-45-9

分子式

C₈H₁₈N₂O₄S

分子量

238.30

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

生物活性

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

性状

Solid

体外研究(In Vitro)

HEPES maintains superhydrophilicity of titanium for at least 3 months and resulted in a continuous retention of bioactivity and osteoconductivity.
HEPES drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, resulting the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

[1]. Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828. [Information]

[2]. Sled? P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66(Pt 4):482-492. [Information]

[3]. Tol MJ, et al. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 2018;14(3):437-449. [Information]

溶解度数据

体外研究:

H₂O : 250 mg/mL (1049.10 mM; Need ultrasonic)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.1964 mL	20.9820 mL	41.9639 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic

搜索质检报告(COA)

产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

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