The IC50 for Torcetrapib determined from the linear portion of the curves (25 to 80 nM) is 52 and 65 nM for the H-HDL and C-LDL cholesteryl ester transfer assays, respectively, using the specific activity-adjusted calculation, and 47 and 61 nM using a single exponential decay function.
Torcetrapib (0, 0.5, 1, 5, and 10 μM) significantly reduced MCF-7 cells growth.
Torcetrapib (0, 1, 5, 10, and 20 μM) does not inducing MCF-7 cells apoptosis.
Torcetrapib (10 μM) binds to CETP with high affinity and down-regulates CETP expression.
Medlife has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay
Cell Line: |
MCF-7 cells |
Concentration: |
0, 0.5, 1, 5, and 10 μM |
Incubation Time: |
5 days |
Result: |
Significantly reduced cell growth. |
RT-PCR
Cell Line: |
MCF-7 cells |
Concentration: |
10 μM |
Incubation Time: |
48 hours |
Result: |
Down-regulated CETP mRNA expression. |