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Amentoflavone

(Synonyms: 穗花杉双黄酮; Didemethyl-ginkgetin)

目录号: PC13809 纯度: ≥98%

穗花杉双黄酮 (Amentoflavone) 是一种天然的双黄酮类化合物，具有很多生物活性，包括抗炎、抗氧化、神经保护等作用。

CAS No. :1617-53-4

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中文名称

Amentoflavone

中文别名

穗花杉双黄酮;穗花双黄酮;阿曼托双黄酮;穗花杉双黄酮(标准品);阿曼托黄素;阿曼托黄酮;穗花杉双;穗花杉双黄酮阿曼托黄酮;穗花杉双黄酮植物提取物,标准品,对照品;乙二胺四乙酸三铵;阿曼托黄酮对照品;布枯草提取物;黄酮(P);穗花杉双黄酮标准品;穗花杉双黄酮,阿曼托黄酮;穗花杉双黄酮, 来源于杉木;穗花杉双黄酮(分析标准品);AMENTOFLAVONE 穗花杉双黄酮;穗花杉双黄酮；1617-53-4；Amentoflavone；HPLC≥98%； 20mg；50mg；100mg；1g；5g ；标准品；对照品

英文名称

Amentoflavone

英文别名

Amentoflavone;DIDEMETHYL-GINKGETIN;I3,II8 BIAPIGENIN;4',4''',5,5'',7,7''-HEXAHYDROXY-3''',8-BIFLAVONE;3',8''-BIAPIGENIN;amenthoflavone;AMENTOFLACONE;AMENTOFLAVONE(SH);4H-1-Benzopyran-4-one,8-[5-(5,7-dihydroxy-4-oxo-4H-1-benzopyran-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-;AMENTOFLAVONE(P) PrintBack;AMentoflavone, froM CunninghaMia lanceolata;AMENTO;I3′,II8-Biapigenin;Tridemethylsciadopitysin;[ "" ];AMENTOFLAVONE hplc;AMentotaxus biflavone;AMENTOFLAVONE WITH HPLC;MLS000574827;9I1VC79L77;SMR000156235;8-[5-(5,7-dihydroxy-4-oxo-chromen-2-yl)-2-hydroxy-phenyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)chromen-4-one;8-[5-(5,7-dihydroxy-4-oxo-4H-chromen-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one;8-[5-(5,7-dihydroxy-4-oxochromen-2-yl)-2-hydroxyphenyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)chromen-4-one;8-(5-(5,7-dihydro

Cas No.

1617-53-4

分子式

C₃₀H₁₈O₁₀

分子量

538.46

包装储存

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

详情描述

穗花杉双黄酮 (Amentoflavone) 是一种天然的双黄酮类化合物，具有很多生物活性，包括抗炎、抗氧化、神经保护等作用。

生物活性

Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase.

性状

Solid

体外研究(In Vitro)

Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells.
Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC₅₀ value of 100 μM at 48 h.
Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase.
Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF-?B activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells.
Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	U-87 MG cells
Concentration:	0, 50, 75, 100, 200 μM
Incubation Time:	48 h
Result:	Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC₅₀ value of 100 μM at 48 h.

Apoptosis Analysis

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 μM
Incubation Time:	48 h
Result:	Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.

Western Blot Analysis

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 μM
Incubation Time:	48 h
Result:	Significantly reduced NF-?B activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.

体内研究(In Vivo)

Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse.

Medlife has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-6 weeks, 28-32 g, kunming mice
Dosage:	25 mg/kg
Administration:	P.o.; once a day for 3 consecutive days
Result:	Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.

运输条件

Room temperature or refrigerated transportation.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

结构分类

来源

参考文献

[1]. Zhang Z, et al. Amentoflavone protects hippocampal neurons: anti-inflammatory, antioxidative, and antiapoptotic effects. Neural Regen Res. 2015 Jul;10(7):1125-33. [Information]

[2]. Woo ER, et al. Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB activation in macrophages. Pharmacol Res. 2005 Jun;51(6):539-46. [Information]

[3]. Yen TH, et al. Amentoflavone Induces Apoptosis and Inhibits NF-?B-modulated Anti-apoptotic Signaling in Glioblastoma Cells. In Vivo. 2018 Mar-Apr;32(2):279-285. [Information]

[4]. Hwang JH, et al. Antibacterial effect of amentoflavone and its synergistic effect with antibiotics. J Microbiol Biotechnol. 2013;23(7):953-8. [Information]

[5]. Hanrahan JR, et al. Semisynthetic preparation of amentoflavone: A negative modulator at GABA(A) receptors. Bioorg Med Chem Lett. 2003 Jul 21;13(14):2281-4. [Information]

溶解度数据

体外研究:

DMSO : 125 mg/mL (232.14 mM; Need ultrasonic)

配制储备溶液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8571 mL	9.2857 mL	18.5715 mL
5 mM	0.3714 mL	1.8571 mL	3.7143 mL
10 mM	0.1857 mL	0.9286 mL	1.8571 mL

产品不同，其溶解度不同。建议根据产品选择合适的溶剂配制储备溶液；配成溶液后，建议分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，建议在 6 个月内使用，-20°C 储存时，建议在 1 个月内使用。

体内研究:

建议根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都建议先按照 体外研究 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

建议依照次序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.64 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH?O 中，得到澄清透明的生理盐水溶液
2.

建议依照次序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (4.64 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (4.64 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

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产品使用指南

Data Sheet

1：一般建议：溶解度为Medlife测试数据，可能与文献描述存在差异。这是由于生产工艺和批次不同产生的正常现象。为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同批次产品溶解度各有差异，仅做参考，具体以实验方案为准。

2：储存条件：粉末-20°C一般情况可以保存3年，溶于溶剂-80°C一般情况可以保存1年。不同产品及不同批次产品可能存在差异，请细致阅读产品信息，并辅助参考相关文献描述。

Amentoflavone

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