Retro-2 是一种内体蛋白质-高尔基体逆行运输的选择性抑制剂。Retro-2 是一种埃博拉病毒 (EBOV) 感染抑制剂，在 HeLa 细胞中的EC₅₀ 为 12.2 µM。Retro-2 还可诱导细胞自噬 (autophagy)。

Retro-2 is a selective inhibitor of retrograde protein trafficking at the endosome-trans-Golgi network interface. Retro-2 is an ebolavirus (EBOV) infection inhibitor with an EC 50 of 12.2 μM in HeLa cells. Retro-2 induces cell autophagy.

Solid

EC50: 12.2 μM (Ebolavirus)

Retro-2 (1 μM; 1-4 hours) induces autophagy in GFP-LC3-expressing HeLa cells and promoted the dramatic cytoplasmic accumulation of large autophagosomes.
Retro-2 (1 μM; 0.5-4 hours) treatment hows an increase over time of the abundance of LC3-II protein in cells.
Retro-2 impairs the trafficking between autophagosomes and lysosomes. Retro-2 abolishes autolysosomes formation.
Retro-2 (20 μM; pretreatment for 30 min) inhibits HeLa cell intoxication by ricin, Shiga-like toxins 1 (Stx1) and 2 (Stx2).
Retro-2 blocked invasion of cells by the intracellular parasite Leishmania , as well as replication of non-enveloped viruses, including polyoma-, papilloma-, and adeno-associated viruses. has not independently confirmed the accuracy of these methods. They are for reference only.

Retro-2 (2-200 mg/kg; i.p; daily; for 21 days) treatment clearly protects from lethal nasal exposure to ricin in mice. 200 mg/kg of Retro-2 fully protects mice against ricin challenge. has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model:

Room temperature in continental US; may vary elsewhere.

4°C, protect from light In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

[1]. Bahne Stechmann, et al. Inhibition of retrograde transport protects mice from lethal ricin challenge. Cell. 2010 Apr 16;141(2):231-42.
[2]. Valérie Nicolas, et al. Small Trafficking Inhibitor Retro-2 Disrupts the Microtubule-Dependent Trafficking of Autophagic Vacuoles. Front Cell Dev Biol. 2020 Jun 18;8:464.

In Vitro: DMSO : 250 mg/mL (780.25 mM; Need ultrasonic)配制储备液