4°C, sealed storage, away from moistur In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
产品详情
MitoSOX Red 是一种特异性靶向活细胞线粒体的新型荧光探针,具细胞膜渗透性,且能快速和选择性结合线粒体。MitoSOX Red 与细胞内线粒体结合后,会被 ROS 氧化产生很强的红色荧光。MitoSOX Red 可以特异性检测超氧化物,对其他 ROS 或 RNS 不敏感。激发/发射波长: 510/580 nm。
生物活性
MitoSOX Red is a novel fluorescent probe that specifically targets mitochondria in living cells. MitoSOX Red is permeable to cell membrane and can bind mitochondria rapidly and selectively. MitoSOX Red combines with mitochondria in cells, it will be oxidized by ROS to produce strong red fluorescence. MitoSOX Red can detect superoxide specifically and is not sensitive to other ROS or RNS. Excitation/emission wavelength: 510/580 nm..
性状
Solid
体外研究(In Vitro)
1. 工作液配制:1.1 将溶液恢复至温室温,瞬时离心使其集中至管底。1.2 利用培养基或合适的缓冲液 (如 PBS) 将 1 mM 储存液稀释至工作浓度,推荐工作液浓度为 50-100 nM;注:请根据实际情况调整 MitoSOX Red 工作液浓度,且现用现配。2. 细胞染色2.1 悬浮细胞:离心收集细胞,加入 PBS 洗涤两次,每次 5 分钟。贴壁细胞:弃去培养基,加入胰蛋白酶消化细胞。离心弃去上清后,加入 PBS 洗涤两次,每次 5 分钟。2.2 加入 1 mL MitoSOX Red 工作液,室温孵育 5-30 分钟。2.3 400 g,4℃ 离心 3-4 分钟,弃去上清。2.4 加入 PBS 洗涤细胞两次,每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后,在荧光显微镜或流式细胞仪下检测。 has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moistur In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
参考文献
[1]. Price OT, et al. Quantitative fluorescence of 5-FU-treated fetal rat limbs using confocal laser scanning microscopy and Lysotracker Red. Cytometry A. 2003;53(1):9-21.[2]. Fengjiao Jin, et al. The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility. Cancer Biol Med. 2019 Feb;16(1):38-54.
溶解度数据
In Vitro: DMSO : 150 mg/mL (197.45 mM; Need ultrasonic)配制储备液