ONPG|369-07-3|(2-Nitrophenyl β-D-galactopyranoside)-陌孚医药/Medlife

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ONPG (Synonyms: 2-Nitrophenyl β-D-galactopyranoside)

目录号: PL14073 纯度: ≥99%

CAS No. :369-07-3

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中文名称	ONPG
中文别名	2-氟代-2-去氧-D-葡萄糖;邻硝基苯-β-D-半乳糖苷;2-硝基苯基-b-D-半乳糖苷;2-硝基苯-beta-D-半乳糖苷;2-硝基苯基-β-D-吡喃半乳糖苷;邻硝基苯-β-D-吡喃半乳糖苷;2-硝基苯基β-D-吡喃半乳糖苷[β-D-牛乳糖用培养基];2-硝基苯基 β-D-吡喃半乳糖苷;2-硝基苯基-beta-D-呋喃半乳糖苷;2'-硝基苯基β-D-吡喃半乳糖苷;2-硝基苯基-β-D-吡喃半乳糖苷(ONPG);2-硝基苯基-β-D-呋喃半乳糖苷;ONPG 2-硝基苯-beta-D-半乳糖苷;ONPG 邻硝基苯-β-D-吡喃半乳糖苷;邻硝基-β-D-吡喃半乳糖苷;邻硝基苯-β-D-半乳糖苷(ONPG);邻硝基酚β－D-半乳糖昔;邻硝基酚β－D-半乳糖昔ONPG;1-硝基丙烷;2-硝基苯-β-D-吡喃半乳糖苷;邻硝...;邻硝基苯基-b-d-半乳吡喃糖苷;邻硝基苯基-β-D-半乳糖苷;邻硝基苯基-β-D-吡喃半乳糖苷;邻硝基苯基-β-D-水解吡啶;磷硝基苯-β-D-吡喃半乳糖苷
英文名称	ONPG
英文别名	2-Nitrophenyl beta-D-Galactopyranoside;ONPG o-Nitrophenyl-?-D-galactoside;ONPG;2-Nitrophenyl β-D-galactopyranoside (ONPG);2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase];2-Nitrophenyl β-D-Galactopyranoside;2-Nitrophenyl-beta-D-galactopyranoside;2-Nitrophenyl-β-D-Galactopyranoside;NITROPHENYL-B-D-GALACTOPYRANOSIDE, 2-(RG);o-Nitrophenyl β-D-Ga;o-Nitrophenyl β-D-Galactopyranoside;O-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE ULTRA PURE GRADE;O-NITROPHENYL-β-D-GALACTOPYRANOSIDE (oNPG);1-Nitropropane;2-Nitrophenyl beta-D-galactoside;o-Nitrophenyl beta-D-galactopyranoside;1-O-[O-NITROPHENYL]-BETA-D-GALACTOPYRANOSE;KUWPCJHYPSUOFW-YBXAARCKSA-N;(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol;2-Nitrophenyl b-D-glucopyranoside;2-Nitrophenyl b-D-galactopyranoside;.beta.-D-Galactopyranoside, 2-nitrophenyl;2-Nitrophenyl β-D-galactopyranoside;o-Nitrophenyl β-D-galactopyranoside
Cas No.	369-07-3
分子式	C₁₂H₁₅NO₈
分子量	301.25
包装储存	Powder -20°C 3 years；4°C 2 years

产品详情	ONPG 是一种比色和分光光度计底物，用于检测 β-半乳糖苷酶活性。
生物活性	ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
性状	Solid
体外研究(In Vitro)	The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s and 2.7±0.3 mM for ONPG, and 264.2±2.1 s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 smM) is 4.6-fold higher than that for lactose (37.2 smM), which clearly demonstrated that the catalytic effi
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Powder -20°C 3 years；4°C 2 years
参考文献	[1]. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237. doi: 10.1186/1471-2180-13-237.

溶解度数据	In Vitro: H₂O : 8.33 mg/mL (27.65 mM; ultrasonic and warming and heat to 60°C)配制储备液

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